Confocal Microscopy Image Gallery

Bovine Pulmonary Artery Endothelial Cells (BPAE Line)

BPAE Cells

GPP130 is a transmembrane protein localized to the cis-Golgi. The culture of bovine pulmonary artery endothelial cells presented in this confocal image was immunofluorescently labeled with anti-GPP130 rabbit monoclonal primary antibodies followed by goat anti-rabbit Fab fragments conjugated to Alexa Fluor 488 (yielding green fluorescence). In addition, the specimen was stained for DNA in the nucleus with the red-absorbing dye TO-PRO-3. Images were recorded with a 60x oil immersion objective using a zoom factor of 4.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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