Confocal Microscopy Image Gallery

Embryonic Swiss Mouse Fibroblast Cells (3T3 Line)

3T3 Cells

The isolated 3T3 cell presented in the digital image above was resident in an adherent culture stained for F-actin with Alexa Fluor 546 conjugated to phalloidin, and for DNA with the red-absorbing dye TO-PRO-3. In addition, the culture was immunofluorescently labeled with Alexa Fluor 488 conjugated to antibodies that target alpha-tubulin, a major component of the microtubule network. Images were recorded with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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