In order to visualize the microtubules present in a culture of Indian Muntjac fibroblasts, the cells were immunofluorescently labeled with anti-tubulin mouse monoclonal primary antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. In addition, the cells were labeled for mitochondria with MitoTracker Red CMXRos, a derivative of X-rosamine, and DNA in the nucleus with the far-red fluorescent probe DRAQ5. Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles, with the exception of DRAQ5, which was pseudocolored cyan.