Confocal Microscopy Image Gallery

Indian Muntjac Deer Skin Fibroblast Cells

Indian Muntjac Deer Skin Fibroblast Cells

The adherent monolayer Indian Muntjac cell culture shown in this confocal image was labeled for the cytoskeletal filamentous actin and intracellular mitochondrial networks with Alexa Fluor 488 conjugated to phalloidin and MitoTracker Red CMXRos, respectively. Nuclei present in the fibroblasts were counterstained with the far-red fluorescent DNA-probe, DRAQ 5 (pseudocolored cyan). Images were recorded on a FluoView FV1000 with a 60x oil immersion objective using a zoom factor of 2.5 and sequential scanning with the 488-nanometer spectral line of an argon-ion laser, the 543-nanometer line from a green helium-neon laser, and the 633-nanometer line of a red helium-neon laser. During the processing stage, the image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles unless otherwise noted above.

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